Lead (Pb) accumulation in rice and its impact on DNA stability

Abstract

Heavy metal contamination, notably lead (Pb), poses a threat to plants and, consequently, human health through the food chain. This study investigates Pb accumulation in rice and how it affects the rice DNA. The rice (Oryza sativa) samples were cultivated in the soil supplemented with Pb at concentrations of 0, 15, 30, 60, and 120 mg/kg. Then the samples were harvested and analyzed for Pb accumulation and DNA alterations using the PCR amplification profiles measured through genomic template stability (GTS). The results demonstrate a Pb concentration hierarchy (root > stem > leaves), with Bioconcentration Factor (BCF) and Translocation Factor (TF) rising when soil Pb contents are supplemented. For DNA alterations, observed GTS values ranged from 23.3 to 76.67%, revealed a general decline with increasing Pb. This correlation assured the influence of Pb on rice DNA stability. Our findings suggest that heavy metal concentration, particularly Pb, has a direct influence on the integrity of rice DNA. Understanding these dynamics is vital for unraveling the complexities of heavy metal-induced genetic changes in plants and their potential implications for food safety and environmental health.