Sauropus species containing eudesmin and their DNA profile

Authors

  • Nuttapong Sawasdee Genetics and Environmental Toxicology Research Group, Khon Kaen University, Thailand Author
  • Arunrat Chaveerach Genetics and Environmental Toxicology Research Group, Khon Kaen University, Thailand Author
  • Tawatchai Tanee Faculty of Environment and Resource Studies, Mahasarakham University, Thailand Author
  • Kittiya Silawong Suwannakud Genetics and Environmental Toxicology Research Group, Khon Kaen University, Thailand Author
  • Pornpimol Ponkham Faculty of Science and Technology, Mahasarakham Rajabhat University, Thailand Author
  • Runglawan Sudmoon Faculty of Law, Khon Kaen University, Thailand Author

DOI:

https://doi.org/10.35495/

Keywords:

Barcode, ISSR fingerprint, Sauropus species, Eudesmin, Breynia species

Abstract

Some Sauropus and Breynia species were investigated for phytochemicals as well as their DNA profile for genetic relationships. S. bicolor and S. thorelii are important species that contain eudesmin concentrations of 36.445 mg and 32.190 mg from 2 g fresh leaves and 1.5 g dried leaves, respectively, and 10.620 mg from 2 g dried leaves was revealed via high-performance liquid chromatography (HPLC) analysis. The genetic relationships calculated via inter simple sequence repeat (ISSR) dendrogram construction, as shown by similarity indices, revealed Breynia and Sauropus separation accuracies, with S values of 0.71-0.81 between genera, S values of 0.76-0.85 at the interspecific level of Breynia and S values of 0.69-0.80 in the Sauropus species, in agreement with the criteria for category distinction. Barcodes with rpoB and trnH-psbA spacer regions can be used to identify some species of the two studied genera.

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Published

30-09-2019

Issue

Vol. 7 No. 03 (2019): Asian Journal of Agriculture and Biology

Section

Articles