Hepatoprotective Effect of Methanolic Extract of Monotheca buxifolia against Isoniazid and Rifampicin Induced Hepatotoxicity

Abstract

The objective of the present study to check free-radical-scavenging and hepatoprotective properties of methanolic extract of Monotheca buxifolia (Falc.) A. DC. aerial parts against isoniazid and rifampicin induced hepatotoxicity in male rats. Results exhibited that methanolic extract and all three sub-fractions (hexane, chloroform and ethyl acetate) of M. buxifolia displayed greater free-radical activity, total phenolic and flavonoid content. However, ethyl-acetate followed by chloroform sub fractions showed the better total phenolic and total flavonoid content. To assess hepatoprotective activity of methanolic extract of M. buxifolia, four groups of the rate were made. Group 1 served as a control, group 2 served as hepatotoxic group (isoniazid and rifampicin orally each 50 mg/kg), group 3 served as standard group received silymarin (200 mg/kg), and 4 served as extract treatment groups, received 500 mg/kg methanolic extract. The results suggested that level of liver markers (total bilirubin, alkaline phosphatases, alanine aminotransferases and aspartate aminotransferases) significantly elevated in group 2. The methanolic extract significantly prevented isoniazid-rifampicin by decreasing raised level of liver markers of Group 4, and also improved histopathological profile of liver. The results indicated the protective effect of methanolic extract of M. buxifolia against liver injury, which might be ascribed to presence of high level of phenolics and flavonoid along with high free-radical activity in the plant extract.