The effects of temperature, pH, and media on mycelium growth of Isaria tenuipes (Peck.) Samson (DL0099) from Lang Biang Mountain, Lam Vien Plateau, Vietnam

Abstract

Isaria tenuipes (Peck.) Samson is an entomopathogenic fungus that has potential in pharmacology and biocontrol. The study aimed to determine the effects of temperature, pH, and media on mycelium growth of Isaria tenuipes (Peck.) Samson (DL0099) from Lang Biang Mountain, Lam Vien Plateau, Vietnam. In this study, we used the single-factor method to culture DL0099 mycelium on both surface liquid and agar media. The results indicated that the optimal temperature for the mycelium growth was 20~25 ^oC. This mushroom mycelium can tolerate temperatures at 35 ^oC for 8 days. The initial media pH range of 6~9 was found to be the most favorable to mycelia growth. Sabouraud’s agar (SA) and Sabouraud’s dextrose agar plus yeast (SDYA) media were the optimal agar media for the mycelium growth rate. Sabouraud broth (S) medium was the most suitable medium for mycelia biomass production in surface liquid culture. Yeast malt agar (YMA), malt agar (MEA), maltose agar (MA), and malt extract yeast agar (MYA) media were suitable for fruit body formation. Our results provided optimal temperature, pH, and media conditions for Isaria tenuipes (DL0099) mycelium growth on surface liquid and agar media. They lay the basis for effective propagation, biomass production, and fruit body formation of the Vietnam native Isaria tenuipes (Peck.) Samson.