The epidemiology of drug resistance in Streptococcus species isolated from yaks in Tibet

Authors

  • Zixuan Li 1Department of Animal Science, Xizang Agriculture and Animal Husbandry University, Key Laboratory for Prevention and Control of Hydatid Disease in Tibet (Co-constructed by Ministry and Province), Linzhi, Tibet 860000, China Author
  • Jiayan Huang Department of Animal Science, Xizang Agriculture and Animal Husbandry University, Key Laboratory for Prevention and Control of Hydatid Disease in Tibet (Co-constructed by Ministry and Province), Linzhi, Tibet 860000, China Author
  • Zhanchun Bai Department of Animal Science, Xizang Agriculture and Animal Husbandry University, Key Laboratory for Prevention and Control of Hydatid Disease in Tibet (Co-constructed by Ministry and Province), Linzhi, Tibet 860000, China Author
  • Zhiyang Zheng Department of Animal Science, Xizang Agriculture and Animal Husbandry University, Key Laboratory for Prevention and Control of Hydatid Disease in Tibet (Co-constructed by Ministry and Province), Linzhi, Tibet 860000, China Author
  • Yangla Bianba Department of Animal Science, Xizang Agriculture and Animal Husbandry University, Key Laboratory for Prevention and Control of Hydatid Disease in Tibet (Co-constructed by Ministry and Province), Linzhi, Tibet 860000, China Author
  • Ci Pu Lhasa Pratacultural Research Institute, Lhasa, Tibet 850000, China Author
  • Sizhu Suolang Department of Animal Science, Xizang Agriculture and Animal Husbandry University, Key Laboratory for Prevention and Control of Hydatid Disease in Tibet (Co-constructed by Ministry and Province), Linzhi, Tibet 860000, China Author

DOI:

https://doi.org/10.35495/

Keywords:

Yaks, Streptococcus, Isolation and identification, Susceptibility test, Resistance analysis

Abstract

This study aimed to investigate the antibiotic sensitivity and resistance profiles of Streptococcus isolated from diarrhea in yaks in Tibetan regions. We successfully isolated and identified Streptococcus from fecal samples using morphological analysis and PCR amplification. Subsequent bacterial susceptibility tests and resistance gene detection revealed that the overall isolate rate of Streptococcus from yaks in Tibet was 57.68%, with Changdu recording the highest (71.43%) and Naqu the lowest (34.09%). The bacteria exhibited significant resistance to macrolides, particularly Erythromycin (52.86%) and Midecamycin (52.14%), β-Lactam resistance, including Ceftazidime, Cephazolin, and Carbenicillin, was the lowest at 7.14%, 2.15%, and 7.14%, respectively. Among the 140 isolates, 37 distinct resistance patterns were identified, with ERY/MID being the most prevalent for Macrolides. The detection rates for the resistance genes were as follows: erma (38.36%), ermb (65.20%), mefa (28.93%) for Macrolides; tetk (13.42%), tetl (14.47%), teto (11.53%), and tetm (20.34%) for Tetracyclines; and pbp2b (5.24%) for β-lactams. The conformity between resistance phenotypes and genes was high, with 82.83% for Tetracyclines, 89.14% for Macrolides, and 95.80% for β-lactams. These findings indicate that Streptococcus in yaks exhibits a predominant resistance to macrolides, accompanied by multiple resistance patterns, with the ERY/MID pattern dominant.

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Published

31-01-2025

Issue

Online First (Future Issue)

Section

Articles