1Department of Biomedicine, Karaganda Medical University, Karaganda, The Republic of Kazakhstan
2Department of Pharmacy, Karaganda Medical University, Karaganda, The Republic of Kazakhstan
3Department of Informatics and Biostatistics, Karaganda Medical University, Karaganda, The Republic of Kazakhstan
Abstract
The aim of this study is to investigate the effects on the nervous system of the use of Stachys sieboldii root powder, in growing male rats on a high fat and sucrose (HFHS) diet. The animals were divided into three groups: Intact – normal rodent chow, HFHS – high fat and sucrose diet, HFHS + Stachys – high fat and sucrose diet with Stachys supplementation. After 30 days of the experiment, the animals were subjected to the “Open Field” test to study the changes in behavioural responses. Biochemical studies of changes in the concentration of lipid peroxidation products in brain homogenates of growing rats were also carried out. Growing males on the HFHS diet exhibit altered nervous system functioning. Animals show apathy, their motor activity and indicators of exploratory behaviour are reduced and the level of emotional reactivity increases. On the part of biochemical changes – the level of all indicators of lipid peroxidation increases significantly. The introduction of Stachys root powder reduces the negative effect of the diet on the organism of animals – they significantly increase the indicators of exploratory behaviour, increase locomotor activity and reduce emotional reactivity, as well as there is a decrease in the concentration of all products of free-radical oxidation in the brain homogenate. Our study showed that diets high in fat and sucrose had negative effects on growing male rats. The introduction of Stachys sieboldii root powder into the diet improved both behavioural response scores and antioxidant processes in the brain.
Keywords: Stachys sieboldii, Open field, Lipid peroxidation, Conjugated dienes, Ketodienes
