Growth and biochemical composition of Spirulina platensis dry biomass in diluted monosodium glutamate waste waters
Wardah Wardah1, Finalah Nurhayati2, Mery Maria Magdalena2, Nur Fazilah2, Tatang Sopandi2*
1Study Program of Agroindustry, Faculty of Vocational, University of 17 Agustus 1945 Surabaya. Jl. Semolowaru 45, Surabaya, East Java, Indonesia
2Study Program of Biology, Faculty of Science and Technology, University of PGRI Adi Buana, Surabaya. Jl. Dukuh Menanggal XII, 60234, Surabaya, East Java, Indonesia
Abstract
The use of low-cost culture media is important for the development of large-scale Spirulina platensis production. This study aims to investigate the effects of using diluted monosodium glutamate wastewater (MSGW) as a culture medium on the growth and biochemical composition of S. platensis dry biomass. Nitrogen and phosphorus uptake by S. platensis from MSGW media was also investigated in this study. Four concentrations of MSGW have been used in this study, namely 1.5, 2.5, 3.5 and 4.5 mL/L and Walne medium was used as a control. All media were adjusted at pH 9 and S. platensis culture was carried out for 14 day at 36.0oC, 77% relative humidity, 5000 lux light intensity and aeration rate at 0.7 L/min. The results of this study have indicated that the growth, protein, lipids, carbohydrates and chlorophyll of S. platensis biomass at 2.5 mL/L MSGW concentrations were not significantly different from Walne medium, but were significantly different from 1.5, 3.5 and 4.5 mL/L MSGW concentrations. The present study have also shown those nitrogen and phosphorus uptakes by S. platensis from 2.5 mL/L MSGW concentration was not significantly different from Walne medium, but were significantly different from 1.5, 3.5 and 4.5 mL/L MSGW concentrations. The present study concluded that S. platensis can be cultivated in MSGW as a medium with an optimum concentration of 2.5 mL/L. This finding will serve as a basic reference for future studies to utilize MSGW for microalgae culture media.
