1Yuxi Zhongyan Seed Company Ltd., Seed Engineering Technology Center of Yunnan Province, Yuxi 653100, China
2Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, China
Abstract
Improvement of seed ageing tolerance is critical for germplasm conservation and field cultivation in tobacco (Nicotiana tabacum L.). However, whether peptides involved in the regulation of seed ageing remains unknown. In this study, a comparative peptidomics analysis conducted to reveal the regulators involving seed ageing in tobacco. Seed vigor was significantly reduced after 5 days artificial ageing treatment compared with that of the unaged seed. A total of 2812 and 4604 peptides identified in the 5 days aged seeds and unaged seeds, respectively; in which 542 peptides were differentially expressed between the aged and unaged seeds. Gene Ontology analysis revealed that the majority precursor proteins of the differentially expressed peptides involved in response to oxygen-containing compound, response to abscisic acid, and response to lipid. Further, the peptides derived from the precursor proteins, such as late embryogenesis abundant protein, peroxiredoxin, catalase, and globulin proteins, involved in seed ageing in tobacco. The identified peptides provide a foundation for the further exploration of seed ageing tolerance in tobacco.
Keywords: Peptidomics, Peptides, Seed ageing, Nicotiana tabacum L., Vigor index
