Characterization of bacterial cellulose produced by Novacetimonas pomaceti KMPG_12, and improving prebiotic property by reducing size using high pressure homogenization
1Biotechnology program, Faculty of Science and Digital Innovation, Thaksin University, Phatthalung Province, Thailand
2Microbial Technology for Agriculture, Food, and Environment Research Center, Faculty of Science and Digital Innovation, Thaksin University, Phatthalung Province, Thailand
Abstract
This study investigates the production and characterization of bacterial cellulose (BC) by Novacetimonas pomaceti KMPG_12, focusing on enhancing its prebiotic properties through size reduction via high-pressure homogenization (HPH). Optimal conditions for BC production were established using Response Surface Methodology (RSM), yielding a maximum dry weight of 9.78 g/L under conditions of 15% glucose, 0.4% peptone, and 4.5% alcohol over a 14-day cultivation period. The produced BC was subjected to HPH at 20,000 psi for eight cycles, significantly reducing fiber size, as confirmed by SEM analysis. The transformation from a compact, net-like structure to a more separated fibrous network at the nanometer scale was observed. FTIR analysis revealed modifications in the BC’s molecular framework post-HPH, indicating a disruption of hydrogen bonds and a transition towards a less crystalline structure. The prebiotic potential of HPH-treated BC was assessed through in vitro experiments with Bacillus amyloliquefaciens, showing a significant enhancement in bacterial proliferation (P=0.0437) compared to untreated BC. This study highlights the potential of BC, particularly when processed to the nanoscale, to serve as a functional ingredient that supports gut health and microbial growth, marking a significant advancement in the development of sustainable and effective prebiotic products.
