Biochemical profile of albino rats with experimentally induced metabolic syndrome fed diet formulations of Cnidoscolus aconitifolius, Gongronema latifolium and Moringa oleifera leaves
Nene Orizu Uchendu1, Chiemekam Samuel Ezechukwu2*, Lawrence Uchenna Sunday Ezeanyika1
1Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria
2Department of Zoology and Environmental Biology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria
Abstract
Metabolic syndrome (MS) has become a globally prevalent disease state, leading to mortality. Plants are a reservoir of compounds that have therapeutic potential and have been proven to be effective in management of a wide range of human and animal diseases. This study therefore, evaluated the effect of diet formulations of Cnidoscolus aconitifolius leaf (CAL), Gongronema latifolium leaf(GLL), and Moringa oleifera leaf (MOL) on some biochemical parameters of experimentally-induced MS in male albino rats. Forty-eight (48) adult male rats of 180-210 g body weight, were separated randomly into eight groups (1 – 8) comprising six rats each. Group 1 was maintained on normal diet. MS was induced in Groups 2 – 8 rats for eight weeks by high fat high carbohydrate (HFHC) diet. Afterwards, group 2 was fed normal rat diet (untreated), while groups 3 to 8 received diets formulated with GLL, CAL, MOL (100g per kg of diet) for eight weeks. Obesity indices, serum lipid profile, liver marker enzymes and antioxidant status were evaluated using standard methods. Significant (p < 0.05) decrease in body weight gain, total cholesterol and triacylglycerols of the treated rats were observed, while high density lipoprotein significantly (p < 0.05) increased compared to the untreated group. Superoxide dismutase and catalase activities significantly (p < 0.05) increased in the treatment groups. Treatment with the herbs showed mild Kupffer cell activation reversing periportal hepatitis induced by the HFHC diet. Results from the study indicate that CAL, GLL and MOL have therapeutic potentials that could be useful in managing components of MS.
Keywords: Antioxidant status, Lipid profile, Metabolic syndrome, High fat high Carbohydrate diet, Kupffer cells
